Molecular recognition technology using phage peptide library for application to molecular design

Development of antiangiogenin peptide using a phage-displayed peptide library.

Angiogenesis is essential for tumor growth and metastasis. Here, we have developed a peptide antagonist of human angiogenin, which is a potent and tumor-associated angiogenic factor. ANI-E peptide was derived from the phage clone, which binds to angiogenin via the disulfide-constrained octapeptide epitope that is displayed on its surface, and is displaced by actin. Disulfide-constrained ANI-E p...

DNA Library Design for Molecular Computation

A novel approach to designing a DNA library for molecular computation is presented. The method is employed for encoding binary information in DNA molecules. It aims to achieve a practical discrimination between perfectly matched DNA oligomers and those with mismatches in a large pool of different molecules. The approach takes into account the ability of DNA strands to hybridize in complex struc...

identification of new peptide ligands for epidermal growth factor receptor using phage display technology

Molecular display technology using yeast--arming technology.

A technology to display proteins or peptides on living organisms has been developed over the last two decades. So-called "Molecular display (Arming) technology" or "Cell surface engineering" has been one of the important tools for analyzing and understanding protein function, or for screening of novel clones from libraries. In addition, it endows cells with novel abilities that cannot be added ...

Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling.

Murine IL-4 is a pleiotropic cytokine with undefined core functional region for eliciting downstream signaling. We used molecular modeling to predict the binding sites recognized by an anti-IL-4-neutralizing mAb (11B.11) and peptide phage display to delineate their makeup. The results of these approaches were confirmed by site-directed mutagenesis analysis. The results suggest that the amino ac...